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Pembalap Tech3 Yamaha Johann Zarco memuncaki sesi latihan bebas pertama MotoGP Brno 2018.
Marc Marquez memimpin diawal sesi latihan FP1 MotoGP Brno 2018, Marquez tetap memimpin hingga pertengahan sesi. Dovizioso kemudian berhasil mengalahkan Marquez dengan 1 m 56.720 detik.
Dovizioso bertahan di depan dengan catatan itu hingga akhir sesi, sampai akhirnya Zarco mengalahkannya dengan catatan 1 m 56.647 detik lenih cepat 0,073s. berikut hasil lengkapnya:
| Pos | Rider | Team | Gap | Laps |
|---|---|---|---|---|
| 1 | Johann Zarco | Tech3 Yamaha | 1m56.647s | 16 |
| 2 | Andrea Dovizioso | Ducati | 0.073s | 16 |
| 3 | Marc Marquez | Honda | 0.181s | 18 |
| 4 | Alvaro Bautista | Aspar Ducati | 0.184s | 17 |
| 5 | Valentino Rossi | Yamaha | 0.241s | 16 |
| 6 | Andrea Iannone | Suzuki | 0.432s | 17 |
| 7 | Dani Pedrosa | Honda | 0.642s | 17 |
| 8 | Stefan Bradl | Honda | 0.644s | 18 |
| 9 | Jack Miller | Pramac Ducati | 0.674s | 17 |
| 10 | Tito Rabat | Avintia Ducati | 0.678s | 17 |
| 11 | Cal Crutchlow | LCR Honda | 0.739s | 17 |
| 12 | Jorge Lorenzo | Ducati | 0.779s | 14 |
| 13 | Hafizh Syahrin | Tech3 Yamaha | 0.781s | 15 |
| 14 | Maverick Vinales | Yamaha | 1.117s | 18 |
| 15 | Danilo Petrucci | Pramac Ducati | 1.291s | 18 |
| 16 | Takaaki Nakagami | LCR Honda | 1.380s | 18 |
| 17 | Alex Rins | Suzuki | 1.385s | 16 |
| 18 | Pol Espargaro | KTM | 1.451s | 16 |
| 19 | Sylvain Guintoli | Suzuki | 1.467s | 18 |
| 20 | Thomas Luthi | MVDS Honda | 1.579s | 16 |
| 21 | Bradley Smith | KTM | 1.591s | 15 |
| 22 | Franco Morbidelli | MVDS Honda | 1.652s | 16 |
| 23 | Scott Redding | Aprilia | 1.850s | 15 |
| 24 | Aleix Espargaro | Aprilia | 1.988s | 15 |
| 25 | Karel Abraham | Aspar Ducati | 2.405s | 13 |
| 26 | Xavier Simeon | Avintia Ducati | 3.216s | 14 |