Dani Pedrosa menempati posisi pertama di FP2 MotoGP Brno.
Rekan setim Pedrosa, Marc Marquez menjadi pembalap tercepat sejak awal FP2 dimulai. Seperti di FP1, Marquez kemudian dikalahkan oleh Andrea Dovizioso. Dovizioso memimpin hanya sebentar, catatan waktunya dikalahkan oleh Pedrosa dengan 1 m 55.976 detik.
Tempat kedua ditempati oleh Danilo Petrucci terpaut 0,123 detik di belakang Pedrosa. Pembalap Nieto Ducati Alvaro Bautista berada di urutan ketiga, sementara Tech3 Hafizh Syahrin secara mengejutkan berada diposisi empat dan menjadi pembalap Yamaha tercepat.
Dovizioso berakhir di posisi kelima, diikuti duo Yamahas Maverick Vinales dan Valentino Rossi. Pembalap Avintia Ducati, Tito Rabat, menempati posisi kedelapan, di depan Zarco dan Marquez. Berikut hasil lengkap FP2 MotoGP Brno 2018:
| Pos | Rider | Team | Bike | Time | Gap | Laps |
|---|---|---|---|---|---|---|
| 1 | Dani Pedrosa | Honda | Honda | 1m55.976s | – | 18 |
| 2 | Danilo Petrucci | Pramac Ducati | Ducati | 1m56.099s | 0.123s | 16 |
| 3 | Alvaro Bautista | Aspar Ducati | Ducati | 1m56.144s | 0.168s | 19 |
| 4 | Hafizh Syahrin | Tech3 Yamaha | Yamaha | 1m56.200s | 0.224s | 14 |
| 5 | Andrea Dovizioso | Ducati | Ducati | 1m56.218s | 0.242s | 16 |
| 6 | Maverick Vinales | Yamaha | Yamaha | 1m56.392s | 0.416s | 17 |
| 7 | Valentino Rossi | Yamaha | Yamaha | 1m56.480s | 0.504s | 17 |
| 8 | Tito Rabat | Avintia Ducati | Ducati | 1m56.521s | 0.545s | 18 |
| 9 | Johann Zarco | Tech3 Yamaha | Yamaha | 1m56.535s | 0.559s | 16 |
| 10 | Marc Marquez | Honda | Honda | 1m56.635s | 0.659s | 18 |
| 11 | Andrea Iannone | Suzuki | Suzuki | 1m56.644s | 0.668s | 18 |
| 12 | Alex Rins | Suzuki | Suzuki | 1m56.661s | 0.685s | 19 |
| 13 | Jack Miller | Pramac Ducati | Ducati | 1m56.915s | 0.939s | 16 |
| 14 | Cal Crutchlow | LCR Honda | Honda | 1m57.014s | 1.038s | 17 |
| 15 | Jorge Lorenzo | Ducati | Ducati | 1m57.205s | 1.229s | 18 |
| 16 | Aleix Espargaro | Aprilia | Aprilia | 1m57.417s | 1.441s | 15 |
| 17 | Pol Espargaro | KTM | KTM | 1m57.473s | 1.497s | 15 |
| 18 | Stefan Bradl | Honda | Honda | 1m57.479s | 1.503s | 18 |
| 19 | Franco Morbidelli | MVDS Honda | Honda | 1m57.498s | 1.522s | 15 |
| 20 | Bradley Smith | KTM | KTM | 1m57.506s | 1.530s | 15 |
| 21 | Takaaki Nakagami | LCR Honda | Honda | 1m57.647s | 1.671s | 18 |
| 22 | Thomas Luthi | MVDS Honda | Honda | 1m57.729s | 1.753s | 16 |
| 23 | Scott Redding | Aprilia | Aprilia | 1m57.941s | 1.965s | 13 |
| 24 | Karel Abraham | Aspar Ducati | Ducati | 1m58.127s | 2.151s | 17 |
| 25 | Xavier Simeon | Avintia Ducati | Ducati | 1m58.453s | 2.477s | 16 |
| 26 | Sylvain Guintoli | Suzuki | Suzuki | 1m58.617s | 2.641s | 17 |
